Part:BBa_K4164017:Design

Inductive expression of FXR-ddRFPA1-RXR-ddRFPB1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1964
  Illegal NheI site found at 2232
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 3846
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 454
  Illegal AgeI site found at 1481
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 836
  Illegal SapI.rc site found at 3435
  

We recombined BBa_K4164015 and BBa_K4164014 to plasmid pET-29a+ by homologous recombination.

Design Notes

Inductive expression of FXR-ddRFPA1-RXR-ddRFPB1

Source

This sequence is composed of BBa_K4164015 and BBa_K4164014.